Cyclin B2 (X29.2), CF647 conjugate, 0.1mg/mL

About this product

In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1) . The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC473059-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Ccnb2; CycB2; Cyclin B2; G2 mitotic specific cyclin B2; HsT17299; MGC108931; MGC140694

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CCNB2

Gene ID

9133

NCBI Gene ID

194698

UniProt

O95067

Swiss Prot

O95067

Host

Mouse

Species Reactivity

Human, Mouse, Rat, Xenopus

Reactivity

Cyclin B2

Immunogen

Recombinant full-length Xenopus laevis Cyclin B2 protein

Target Antigen

Cyclin B2

Clonality

Monoclonal

Clone

X29.2

Type

Primary

Source

Animal

Origin

Animal

Applications

IF (published)

Positive Control

HeLa cells. Human cervical tissue (IHC).

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

51 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. PLoS ONE 3(4): e1871. (IF)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647