ORC1 (7F6/1), CF647 conjugate, 0.1mg/mL

About this product

The initiation of DNA replication is a multi-step process that depends on the formation of pre-replication complexes, which trigger initiation. Among the proteins required for establishing these complexes are the origin recognition complex (ORC) proteins. ORC proteins bind specifically to origins of replication where they serve as scaffold for the assembly of additional initiation factors. Human ORC subunits 1-6 are expressed in the nucleus of proliferating cells and tissues, such as the testis. ORC1 and ORC2 are both expressed at equivalent concentrations throughout the cell cycle; however, only ORC2 remains stably bound to chromatin. ORC4 and ORC6 are also expressed constantly throughout the cell cycle. ORC2, ORC3, ORC4 and ORC5 form a core complex upon which ORC6 and ORC1 assemble. The formation of this core complex suggests that ORC proteins play a crucial role in the G1-S transition in mammalian cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC473067-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

HSORC1; MmORC1; Origin Recognition Complex 1 (ORC1) ; Origin recognition complex subunit 1 (yeast homolog) like (ORC1L) ; Origin recognition complex subunit 1 like (S. cerevisia; Origin recognition complex subunit 1 like; PARC1; Replication control protein 1

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

ORC1L

Gene ID

4998

NCBI Gene ID

17908

UniProt

Q13415

Swiss Prot

Q13415

Host

Mouse

Species Reactivity

Human

Reactivity

ORC1

Immunogen

Recombinant full-length human ORC1 protein

Target Antigen

ORC1

Clonality

Monoclonal

Clone

7F6/1

Type

Primary

Source

Animal

Origin

Animal

Positive Control

HeLa cells.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

120 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647