HPV16 E2 (Human Papilloma Virus 16) (TVG 261), CF647 conjugate, 0.1mg/mL

About this product

The human papilloma virus (HPV) family of DNA tumor viruses includes HPV16, a strain that is responsible for the largest number of cases of cervical cancers linked to the family. HPV16E1 and HPV16E2 are proteins that are involved in the regulation of viral DNA replication and are important for infected cell homeostasis. HPV16E2 specifically regulates the expression of the E6 and E7 oncoproteins by binding to four sites within the viral long control region, possibly involving interactions with nuclear hormone receptors. Integration of the HPV genome into the host DNA usually disrupts the HPV16E2 gene open reading frames, resulting in an overexpression of E6 and E7 genes, an event that may lead to the malignant transformation of cervical cancer. HPV16E2 is also able to induce apoptotic cell death via two pathways: the first through the binding of p53 and the second through the binding of the viral genome. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC473073-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

HPV16 regulatory protein E2; Human papilloma virus 16 E2; Human papilloma virus 16 regulatory protein E2; Human papillomavirus type 16 E2; Human papillomavirus type 16 regulatory protein E2; Regulatory protein E2

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

Not Applicable

UniProt

Not Applicable

Swiss Prot

Not Applicable

Host

Mouse

Species Reactivity

HPV-16

Reactivity

HPV16 E2

Immunogen

Raised against Vaccinia-E2 followed by intravenous injection of the maltose binding protein MBP-E2

Target Antigen

HPV16 E2

Clonality

Monoclonal

Clone

TVG 261

Type

Primary

Source

Animal

Origin

Animal

Applications

IF (published) |WB (published)

Positive Control

HPV-16 infected cells. Cervical tissue.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

43 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Virol(2003) 77(3): 2021-2028. (WB) J Virol (2017) 91(5): e02305-16. (IF) Virology (2018) 521: 62-68. (WB)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647