APC / Adenomatous Polyposis Coli / FAP (Tumor Suppressor) (ALi 12-28), CF647 conjugate, 0.1mg/mL

About this product

Adenomatous Polyposis Coli (APC) is a protein with many cellular functions. It is a tumor suppressor and negative regulator of Wnt signaling. APC has roles in cell adhesion and cell migration. Mutations in APC are assuciated with several diseases. The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner's syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC473076-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Adenomatous Polyposis Coli; APC; CC1; Deleted in polyposis 2.5; Protein Phosphatase 1, Regulatory Subunit 46; DP2; DP3

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

APC

Gene ID

324

NCBI Gene ID

158932

UniProt

P25054

Swiss Prot

P25054

Host

Mouse

Species Reactivity

Human

Reactivity

Adenomatous Polyposis Coli

Immunogen

Recombinant fragment of human APC protein (around aa 1-433)

Target Antigen

Adenomatous Polyposis Coli

Clonality

Monoclonal

Clone

ALi 12-28

Type

Primary

Source

Animal

Origin

Animal

Applications

IF (published) |IHC, frozen (published) |IP (published) |WB (published)

Positive Control

COLO 320DM whole cell lysate. HCT116 cells. Tonsil, placenta, breast or colon.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

66 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. PNAS USA (1998) 95:3122-3127. (WB, IF, epitope mapping) EMBO Rep (2005) 6:184-190. (IF) BMC Cell Biol (2006) 7: 3. (IF, WB) Int J Mol Med (2008) 21:19-31. (IHC, frozen) J Biol Chem (2012) 287(34): 28552-28563. (IP, WB)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647