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Product details

RNA Polymerase II CTD Repeat YSPTSPS (Phospho S5) (CTD 8A7), 1mg/mL

Catalog number BNUM3099-50

Price for BNUM3099-50

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Size / count 1x 50 µL

About this product

RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur. This antibody is available purified, with BSA and azide (0.2 mg/mL) or purified, BSA- and azide-free (1 mg/mL) .

Specifications

Catalog number
BNUM3099-50
Size / count
1x 50 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit RPB1; hRPB220; hsRPB1; POLR2; Polr2a; POLRA; Polymerase (RNA) II (DNA directed) polypeptide A 220kDa; RNA polymerase II subunit B1; RPBh1; RpIILS; RPO2; RPOL2
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
POLR2A
Gene ID
5430
NCBI Gene ID
270017
UniProt
P24928
Swiss Prot
P24928
Host
Mouse
Species Reactivity
Human, Yeast
Reactivity
RNA Polymerase II CTD Repeat YSPTSPS
Immunogen
Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
Target Antigen
RNA Polymerase II CTD Repeat YSPTSPS
Clonality
Monoclonal
Clone
CTD 8A7
Type
Primary
Source
Animal
Origin
Animal
Applications
IHC, FFPE (verified) |WB (verified)
Positive Control
HAP1, K562, PC3, HePG2, NIH3T3 cells. Human testis.
Stability
2 years
Concentration
1 mg/mL
Buffer
PBS, no BSA, no azide
Molecular Weight
192-253 kDa
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgM, kappa
Species
Mus musculus (mouse)
Classification
PBS, no BSA, no sodium azide (CAS 26628-22-8)
Conjugate
Purified, BSA-free

Frequently Asked Questions

What is the catalog number for RNA Polymerase II CTD Repeat YSPTSPS (Phospho S5) (CTD 8A7), 1mg/mL?

The catalog number for RNA Polymerase II CTD Repeat YSPTSPS (Phospho S5) (CTD 8A7), 1mg/mL is BNUM3099-50.

What sizes are available for RNA Polymerase II CTD Repeat YSPTSPS (Phospho S5) (CTD 8A7), 1mg/mL?

RNA Polymerase II CTD Repeat YSPTSPS (Phospho S5) (CTD 8A7), 1mg/mL is available in the following size(s): 1x 50 µL.

Is RNA Polymerase II CTD Repeat YSPTSPS (Phospho S5) (CTD 8A7), 1mg/mL in stock?

RNA Polymerase II CTD Repeat YSPTSPS (Phospho S5) (CTD 8A7), 1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures RNA Polymerase II CTD Repeat YSPTSPS (Phospho S5) (CTD 8A7), 1mg/mL?

RNA Polymerase II CTD Repeat YSPTSPS (Phospho S5) (CTD 8A7), 1mg/mL is manufactured by Biotium.

What is the estimated delivery time for RNA Polymerase II CTD Repeat YSPTSPS (Phospho S5) (CTD 8A7), 1mg/mL?

Estimated delivery time for RNA Polymerase II CTD Repeat YSPTSPS (Phospho S5) (CTD 8A7), 1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for RNA Polymerase II CTD Repeat YSPTSPS (Phospho S5) (CTD 8A7), 1mg/mL?

The safety data sheet for RNA Polymerase II CTD Repeat YSPTSPS (Phospho S5) (CTD 8A7), 1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd029b061270361e101cab/msds/bnum3099-50.pdf.

What is RNA Polymerase II CTD Repeat YSPTSPS (Phospho S5) (CTD 8A7), 1mg/mL used for?

RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The larges

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

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