TIM3 / HAVCR2 / CD366 (Effector T-Cell Marker) (TIM3/3113), CF405S conjugate, 0.1mg/mL

About this product

TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. TIM-3 is expressed on the surface of effector T cells (CD4 Th1 and CD8 Tc1) but not on helper T cells (CD4 Th2 and CD8 Tc2) . In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells. The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling. TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity, regulatory T cell development, and increases in macrophage and neutrophil infiltration into sites of inflammation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC043113-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CD366; HAVR2; Hepatitis A virus cellular receptor 2 (HAVCR2) ; Kidney injury molecule 3 (KIM3) ; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM3; TIMD3

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

HAVCR2

Gene ID

84868

NCBI Gene ID

710500

UniProt

Q8TDQ0

Swiss Prot

Q8TDQ0

Host

Mouse

Species Reactivity

Human

Reactivity

CD366|HAVCR2|TIM3

Immunogen

Recombinant fragment of human TIM3 protein (around aa 22-202) (exact sequence is proprietary)

Target Antigen

CD366 | HAVCR2 | TIM3

Clonality

Monoclonal

Clone

TIM3/3113

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE (verified)

Positive Control

PC3, BT474, HepG2, HDLM-2 or Daudi cells. Tonsil, lymph node or spleen.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

60 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF405S