ALK (Anaplastic Lymphoma Kinase) / CD246 (ALK/3218R), CF647 conjugate, 0.1mg/mL

About this product

The wild-type anaplastic lymphoma kinase (ALK) protein is a 200 kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes) . ALK has been found to be rearranged, mutated, or amplified in a series of tumors. The hybrid gene, NPM-ALK, created by the t (2;5) (p23; q35) chromosomal translocation, encodes part of the nucleolar phosphoprotein nucleophosmin (NPM), joined to the entire cytoplasmic portion of ALK. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80 kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL) . Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein, producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC473218-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

ALK Tyrosine Kinase Receptor; Anaplastic Lymphoma Kinase; ALK/NPM1 fusion gene

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

ALK

Gene ID

238

NCBI Gene ID

654469

UniProt

Q9UM73

Swiss Prot

Q9UM73

Host

Rabbit

Species Reactivity

Human

Reactivity

ALK|CD246

Immunogen

Recombinant human ALK protein fragment (aa200-335)

Target Antigen

ALK | CD246

Clonality

Recombinant Monoclonal

Clone

ALK/3218R

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE (verified)

Positive Control

Anaplastic Large Cell Lymphoma

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

80 kDa (hybrid) ; 200 kDa (wild type)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647