E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) (CDH1/3256), Biotin conjugate, 0.1mg/mL

About this product

Recognizes a protein of 120-80 kDa, identified as E-cadherin. Cadherins comprise a family of Ca2 -dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as -catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNCB3256-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Arc 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CDHE; E-Cad/CTF3; E-cadherin; ECAD; Epithelial cadherin; epithelial calcium dependent adhesion protein; Liver cell adhesion molecule (LCAM) ; Uvomorulin (UVO)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CDH1

Gene ID

999

NCBI Gene ID

461086

UniProt

P12830

Swiss Prot

P12830

Host

Mouse

Species Reactivity

Human

Reactivity

CD324|E-Cadherin

Immunogen

Recombinant fragment of human CDH1 protein (around aa 567-691) (exact sequence is proprietary)

Target Antigen

CD324 | E-Cadherin

Clonality

Monoclonal

Clone

CDH1/3256

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow (verified) |IHC, FFPE (verified)

Positive Control

MCF-7, LS174T, Raji, HT29 or SK-BR3 cells. Prostate or Colon carcinomas.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

120-80 kDa (Mature) ; 135 kDa (Precursor)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

Biotin