Lymphocyte Activation Gene 3 (LAG-3) (Negative Checkpoint Regulator) (LAG3/3261), CF568 conjugate, 0.1mg/mL

About this product

LAG-3 (also called CD223) is a high affinity MHC class II ligand present on the surface of CD4 CD8 T cells and NK cell, with shared homology in structure to CD4 molecules. It has a glutamic acid-proline (EP) repetitive sequence found in other functionally distinct mammalian, parasitic, and bacterial proteins that may influence a conserved biological function. LAG-3 CD4 CD8 T cells can associate with the T cell receptor (TCR) and downregulate TCR signaling in vitro. LAG-3 inhibits CD4-dependent T cell function via its cytoplasmic domain. LAG-3 Lys-468 within a conserved 'KIEELE' motif is essential for interaction with downstream signaling molecules. Furthermore, as a checkpoint inhibitor target, it may be superior to CTLA-4 and PD-1 since both antibodies only activate effector T-cells, whereas an antagonist LAG-3 antibody can both activate T effector cells (by downregulating the LAG-3 inhibiting signal into pre-activated LAG-3 cells) and inhibit induced (i. e. antigen-specific) Treg suppressive activity. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC683261-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CD223; FDC protein; LAG-3

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

LAG3

Gene ID

3902

NCBI Gene ID

409523

UniProt

P18627

Swiss Prot

P18627

Host

Mouse

Species Reactivity

Human

Reactivity

Lymphocyte Activation Gene 3

Immunogen

Recombinant full-length human LAG3 protein

Target Antigen

Lymphocyte Activation Gene 3

Clonality

Monoclonal

Clone

LAG3/3261

Type

Primary

Source

Animal

Origin

Animal

Positive Control

Human tonsil or Hodgkin's lymphoma (IHC-P).

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

70 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF568