Cyclin D1 (G1-Cyclin & Mantle Cell Lymphoma Marker) (CCND1/3548), CF647 conjugate, 0.1mg/mL

About this product

Recognizes a protein of 36 kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t (11; 14) (q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative) . Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC473548-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

B cell CLL/lymphoma 1; B cell leukemia 1; B-cell lymphoma 1 protein; BCL-1 oncogene; CCND1 protein; CCND1/FSTL3 fusion gene; CCND1/IGHG1 fusion gene CCND1/IGLC1 fusion gene; CCND1/PTH fusion gene; G1/S-specific cyclin-D1; Parathyroid adenomatosis 1; PRAD1 oncogene

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CCND1

Gene ID

595

NCBI Gene ID

523852; 667996

UniProt

P24385

Swiss Prot

P24385

Host

Mouse

Species Reactivity

Human

Reactivity

Cyclin D1

Immunogen

Recombinant fragment of human CCND1 (around aa 115-270) protein (exact sequence is proprietary)

Target Antigen

Cyclin D1

Clonality

Monoclonal

Clone

CCND1/3548

Type

Primary

Source

Animal

Origin

Animal

Positive Control

T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. Mantle cell lymphoma, breast or bladder carcinomas.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

36 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2b, kappa

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647