Cytokeratin 5 (KRT5) (Basal, Myoepithelial & Mesothelial Cell Marker) (KRT5/3594), CF405S conjugate, 0.1mg/mL

About this product

This MAb recognizes a protein of 58 kDa, which is identified as Cytokeratin 5 (KRT5) . This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Antibodies to KRT5 identify basal cells of squamous and glandular epithelia, myoepithelia, and mesothelium. Anti-cytokeratin 5 has been reported useful in the differential diagnosis of metastatic carcinoma in the pleura versus epithelioid mesothelioma. Almost all squamous cell carcinomas, half of transitional carcinomas, and many undifferentiated large cell carcinomas express. Anti-KRT5, along with anti-p63, affords a high sensitivity and specificity for squamous differentiation. Myoepithelial cells of the breast, glandular epithelia, and basal cells of the prostate are labeled with anti-KRT5. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC043594-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

58kDa Cytokeratin; DDD1; Epidermolysis Bullosa Simplex 2 (EBS2) ; Keratin 5; Keratin; Type II Cytoskeletal 5; Keratin-5; Type-II Cytoskeletal 5; Type-II keratin Kb5

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

KRT5

Gene ID

3852

NCBI Gene ID

433845

UniProt

P13647

Swiss Prot

P13647

Host

Mouse

Species Reactivity

Human

Reactivity

Cytokeratin 5

Immunogen

Recombinant fragment (around aa 316-590) of human Cytokeratin 5 (KRT5) protein (exact sequence is proprietary)

Target Antigen

Cytokeratin 5

Clonality

Monoclonal

Clone

KRT5/3594

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE (verified)

Field of Research

Cancer, Cytoskeleton

Positive Control

MCF-7 or HeLa cells. Human tonsil, esophagus or bladder tissue (IHC).

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

58 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF405S