Nucleophosmin (Acute Myeloid Leukemia Marker) (rNPM1/1901), CF647 conjugate, 0.1mg/mL

About this product

Recognizes a 33 kDa glycoprotein, identified as Nucleophosmin (NPM) . It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC473807-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

NO38; NPM; NPM1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin (nucleolar phosphoprotein B23 numatrin) ; Nucleophosmin; Nucleophosmin/nucleoplasmin family member 1; Numatrin

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

NPM1

Gene ID

4869

NCBI Gene ID

557550

UniProt

P06748

Swiss Prot

P06748

Host

Mouse

Species Reactivity

Human

Reactivity

Nucleophosmin

Immunogen

Recombinant human NPM1 protein fragment (aa185-287) (exact sequence is proprietary)

Target Antigen

Nucleophosmin

Clonality

Recombinant Monoclonal

Clone

RNPM1/1901

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular (verified) |IHC, FFPE (verified) |WB (verified)

Positive Control

MCF-7, HeLa or A431 cell lysate (WB). Human skin or colon tissue (IHC).

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

33 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647