Human Immunoglobulin Alpha (IgA) Heavy Chain (rHISA43), CF405S conjugate, 0.1mg/mL

About this product

This MAb is specific to heavy chain of IgA and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of Alpha heavy chain. Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through inter-chain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin A (IgA) is the main protein of the mucosal immune system. It is generated by B-cells in gut-associated lymphoid tissues. Daily production of IgA exceeds that of any of the other immunoglobulins. IgA exists mainly in dimers but can also exist as polymers or as monomers. Dimers and polymers contain a joining (J) chain that can be bound by the polymeric immunoglobulin receptor (pIgR) for transportation of the molecule to mucosal surfaces. The most common feature of plasmacytomas, and certain non-Hodgkins lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC043876-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

A2m Marker; Ig alpha 1 Chain C Region; Ig alpha 2 Chain C Region; IGHA1; IGHA2; Immunoglobulin Am1; Immunoglobulin Am2; Immunoglobulin Heavy Constant Alpha 1; Immunoglobulin Heavy Constant Alpha 2

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

IGHA1; IGHA2

Gene ID

3493; 3494

NCBI Gene ID

699841

UniProt

P01876; P01877

Swiss Prot

P01876; P01877

Host

Mouse

Species Reactivity

Human

Reactivity

Immunoglobulin Alpha Heavy Chain

Immunogen

Recombinant full-length human IGHA1 and IGHA2 proteins

Target Antigen

Immunoglobulin Alpha Heavy Chain

Clonality

Recombinant Monoclonal

Clone

RHISA43

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE (verified)

Field of Research

Cancer, Immunology, Inflammation

Positive Control

Daudi, 293T, Raji or hPBL cells. Human tonsil or spleen.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

50-75 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF405S