Human TNFSF11 / RANKL / CD254 Protein, Mouse IgG2a Fc Tag

About this product

Human TNFSF11, Mouse IgG2a Fc Tag (RAL-H52A6) is expressed from human 293 cells (HEK293). It contains AA Gly 64 - Asp 245 (Accession # AAC51762.1).

Specifications

Catalog number

RAL-H52A6-1mg

Size / count

1 mg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is known as a type II membrane protein and is a member of the tumor necrosis factor (TNF) superfamily. RANKL, through its ability to stimulate osteoclast formation and activity, is a critical mediator of bone resorption and overall bone density. Some findings also suggestion some cancer cells, particularly prostate cancer cells, can activate an increase in bone remodeling and ultimately increase overall bone production.[17] This increase in bone remodeling and bone production increases the overall growth of bone metastasizes. The overall control of bone remodeling is regulated by the binding of RANKL with its receptor or its decoy receptor, respectively, RANK and OPG.

Specifications

This protein carries a mouse IgG2a Fc tag at the N-terminus. The protein has a calculated MW of 47.4 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Gly 64 - Asp 245

Host

HEK 293

Target

TNFSF11

Conjugation

Unconjugated

Tag

N-mFc(mIgG2a)

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.1 EU per μg

Purity

95%

Bioactivity

Immobilized Human Osteoprotegerin, His Tag (Cat. No. TNB-H5220) at 2 μg/mL (100 μL/well) can bind Human TNFSF11, Mouse IgG2a Fc Tag (Cat. No. RAL-H52A6) with a linear range of 0.2-2 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

47.4 kDa

Shipping Conditions

Room Temperature

Storage Conditions

-20°C