Kappa Light Chain Recombinant Mouse Monoclonal Antibody [A8H10-R]

About this product

Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two types of light chains designated as kappa and lambda. The gene rearrangement process that generates the immunoglobulin molecule results in either a productive kappa gene or a productive lambda gene. The mechanics of the rearrangement process normally produce approximately twice as many kappa-bearing cells as lambda. However this ratio loses during malignant transformation. The kappa light chain antibody labels kappa light chain expressing B lymphocytes and plasma cells. Other cells may also express kappa light chain due to nonspecific uptake of immunoglobulin. Individual B cells express either kappa or lambda light chains. Monoclonality is generally assumed to be evidence of a malignant proliferation. Paired with lambda, kappa light chain is useful in identifying monoclonality of lymphoid malignancies. Anti-Kappa detects surface immunoglobulin on normal and neoplastic B-cells. In paraffin-embedded tissue, anti-kappa exhibits strong staining of kappa-positive plasma cells and cells that have absorbed exogenous immunoglobulins. When dealing with B-cell neoplasms, the determination of light chain ratios remains the centerpiece. Most B-cell lymphomas express either kappa or lambda light chains, whereas reactive proliferations display a mixture of kappa and lambda positive cells. If only a single light chain type is detected, a lymphoproliferative disorder exists. Monoclonality is determined by a kappa-lambda ratio of greater than or equal to 3:1 or a lambda-kappa ratio greater than 2:1. Tonsil is an appropriate control: Approximately half of the peripheral mantle zone B-cells must show a distinct membranous staining reaction for IgK, while the remaining mantle zone B-cells (which are IgL producing) should be unstained.

Specifications

Catalog number

HA601266

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

HCAK 1 antibody HCAK1 antibody Ig kappa chain C region antibody IGKC antibody IGKCD antibody Immunoglobulin InV antibody Immunoglobulin kappa constant antibody Immunoglobulin kappa constant region antibody Immunoglobulin kappa light chain antibody Immunoglobulin kappa light chain constant region antibody Immunoglobulin KM antibody Kappa 1 immunoglobulin light chain antibody kappa light chain antibody Km antibody MGC111575 antibody MGC62011 antibody MGC72072 antibody MGC88770 antibody MGC88771 antibody MGC88809 antibody

Abbreviation

Immunoglobulin kappa constant Ig kappa chain C region Ig kappa chain C region AG Ig kappa chain C region CUM Ig kappa chain C region EU Ig kappa chain C region OU Ig kappa chain C region ROY Ig kappa chain C region TI IGKC

Swiss Prot

P01834 Human

Cellular Locus

Cell membrane, Secreted.

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant full protein within human IGKC.

Isotype

IgG1

Clone

A8H10-R

Conjugation

Non-conjugated

Type

Recombinant Mouse monoclonal Antibody

Applications

WB, IHC-P

Positive Control

Human plasma lysates, human tonsil tissue, human appendix tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:500-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 12 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes