SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His Tag (MALS verified)

Catalog number SPD-C5228-100ug

Discontinued

Price for SPD-C5228-100ug

Size / count 100 µg

This variant is discontinued and cannot be added to your cart.

About this product

SARS-CoV-2 Spike RBD, His Tag (SPD-C5228) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (Y449H, E484K, N501Y)). The mutations Y449H, E484K, N501Y were identified in the SARS-CoV-2 variant C.1.2.

Specifications

Catalog number: SPD-C5228-100ug
Size / count: 100 µg
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.5 kDa. The protein migrates as 32-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Arg 319 - Lys 537
Host: HEK 293
Target: Spike RBD
Conjugation: Unconjugated
Tag: C-10×His
Source: SARS-CoV-2
Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin: 1.0 EU per μg
Purity: 95%
Bioactivity: Immobilized SARS-CoV-2 Spike RBD (Y449H, E484K, N501Y), His Tag (Cat. No. SPD-C5228) at 5 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-2 ng/mL (QC tested).
Format: Powder
Buffer: PBS, pH7.4
Additives: Trehalose
Molecular Weight: 26.5 kDa
Shipping Conditions: Room Temperature
Storage Conditions: -20°C

Frequently Asked Questions

What is the catalog number for SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His Tag (MALS verified)?

The catalog number for SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His Tag (MALS verified) is SPD-C5228-100ug.

Is SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His Tag (MALS verified) in stock?

SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His Tag (MALS verified) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His Tag (MALS verified)?

SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His Tag (MALS verified) is manufactured by CRB.

What is the estimated delivery time for SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His Tag (MALS verified)?

Estimated delivery time for SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His Tag (MALS verified) is approximately 10 business day(s), depending on your location.

What is SARS-CoV-2 Spike RBD Protein (Y449H, E484K, N501Y), His Tag (MALS verified) used for?

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.