Phospho-eIF4B (S406) Recombinant Rabbit Monoclonal Antibody [JE63-80]

Catalog number HA722149

Price for HA722149

Size / count 100 µL

About this product

Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly- (A) binding protein, increasing its poly- (A) binding activity. Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation. Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002. Recently, Ser406 was identified as a novel phosphorylation site regulated by mitogens, and the phosphorylation of this site is dependent on MEK and mTOR activity. This phosphorylation is shown to be essential for the translational activity of eIF4B.

Specifications

Catalog number: HA722149
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: 2310046H11Rik antibody AL024095 antibody C85189 antibody EIF 4B antibody eIF-4B antibody EIF4B antibody Eukaryotic initiation factor 4B antibody Eukaryotic translation initiation factor 4B antibody IF4B_HUMAN antibody PRO1843 antibody
Abbreviation: EIF4B
Swiss Prot: P23588 Human, Q8BGD9 Mouse, Q5RKG9 Rat
Cellular Locus: cytosol, eukaryotic translation initiation factor 4F complex.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide corresponding to residues surrounding Ser406 of human eIF4B protein.
Isotype: IgG
Clone: JE63-80
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IF-Cell, IHC-P, FC
Positive Control: 293T cell lysate, 293T treated with 100nM Calyculin A for 15 minutes cell lysate, HeLa cell lysate, HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, Jurkat cell lysate, Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, PC-12 cell lysate, PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate, human breast cancer tissue, Jurkat, NIH/3T3, PC-12.
Concentration: 1 mg/mL
Dilution: WB: 1:50,000 ;IF-Cell: 1:5,000-1:20,000 ;IHC-P: 1:1,000 ;FC: 1:5,000
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 69 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for Phospho-eIF4B (S406) Recombinant Rabbit Monoclonal Antibody [JE63-80]?

The catalog number for Phospho-eIF4B (S406) Recombinant Rabbit Monoclonal Antibody [JE63-80] is HA722149.

What sizes are available for Phospho-eIF4B (S406) Recombinant Rabbit Monoclonal Antibody [JE63-80]?

Phospho-eIF4B (S406) Recombinant Rabbit Monoclonal Antibody [JE63-80] is available in the following size(s): 100 µL.

Is Phospho-eIF4B (S406) Recombinant Rabbit Monoclonal Antibody [JE63-80] in stock?

Phospho-eIF4B (S406) Recombinant Rabbit Monoclonal Antibody [JE63-80] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Phospho-eIF4B (S406) Recombinant Rabbit Monoclonal Antibody [JE63-80] used for?

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.