Jarid2 Recombinant Rabbit Monoclonal Antibody [PSH01-32]

About this product

Protein Jumonji is a protein that in humans is encoded by the JARID2 gene. JARID2 is a member of the alpha-ketoglutarate-dependent hydroxylase superfamily. Jarid2 (jumonji, AT rich interactive domain 2) is a protein coding gene that functions as a putative transcription factor. Distinguished as a nuclear protein necessary for mouse embryogenesis, Jarid2 is a member of the jumonji family that contains a DNA binding domain known as the AT-rich interaction domain (ARID). In vitro studies of Jarid2 reveal that ARID along with other functional domains are involved in DNA binding, nuclear localization, transcriptional repression, and recruitment of Polycomb-repressive complex 2 (PRC2). Intracellular mechanisms underlying these interactions remain largely unknown. In search of developmentally important genes, Jarid2 has previously been identified by gene trap technology as an important factor necessary for organ development. During mouse organogenesis, Jarid2 is involved in the formation of the neural tube and development of the liver, spleen, thymus and cardiovascular system. Continuous Jarid2 expression in the tissues of the heart, highlight its presiding role in the development of both the embryonic and the adult heart. Mutant models of Jarid2 embryos show severe heart malformations, ventricular septal defects, noncompaction of the ventricular wall, and atrial enlargement. Homozygous mutants of Jarid2 are found to die soon after birth. Overexpression of the mouse Jarid2 gene has been reported to repress cardiomyocyte proliferation through it close interaction with retinoblastoma protein (Rb), a master cell cycle regulator. Retinoblastoma-binding protein-2 and the human SMCX protein share regions of homology between mice and humans.

Specifications

Catalog number

HA721656

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

JARD2 antibody JARD2_HUMAN antibody JARID2 antibody JMJ antibody Jumonji AT rich interactive domain 2 antibody Jumonji homolog antibody Jumonji like protein antibody Jumonji protein antibody Jumonji/ARID domain containing protein 2 antibody Jumonji/ARID domain-containing protein 2 antibody Protein Jumonji antibody

Abbreviation

JARID2, JMJ

Swiss Prot

Q92833 Human, Q62315 Mouse

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant protein within human Jarid2 aa 501-1,050 / 1,246.

Isotype

IgG

Clone

PSH01-32

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell

Positive Control

NCCIT cell lysate, SH-SY5Y cell lysate, K-562 cell lysate, Jurkat cell lysate, MDA-MB-231 cell lysate, A549 cell lysate, HepG2 cell lysate, HUVEC cell lysate, HeLa cell lysate, HEK-293 cell lysate, human brain tissue lysate, NCCIT, human cervix tissue, human placenta tissue, human thyroid tissue, mouse esophagus tissue.

Concentration

0.95 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:200-1:1,000 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 139 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes