CDT2 Recombinant Rabbit Monoclonal Antibody [JE58-01]

About this product

Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX (DTL) complex, also named CRL4 (CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21 (CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21 (CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX (DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX (DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.

Specifications

Catalog number

HA721871

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Lethal (2) denticleless protein homolog antibody CDW1 antibody DCAF2 antibody DDB1 and CUL4 associated factor 2 antibody Ddb1- and Cul4-associated factor 2 antibody Denticleless homolog antibody Denticleless homolog (Drosophila) antibody Denticleless protein homolog antibody Dtl antibody DTL_HUMAN antibody L2DTL antibody Lethal (2) denticleless protein homolog antibody RA regulated nuclear matrix associated protein antibody RAMP antibody Retinoic acid regulated nuclear matrix associated protein antibody Retinoic acid-regulated nuclear matrix-associated protein antibody

Abbreviation

DTL, CDT2, CDW1, DCAF2, L2DTL, RAMP

Swiss Prot

Q9NZJ0 Human, Q3TLR7 Mouse

Cellular Locus

Nucleus, Nucleus membrane, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Chromosome.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant protein within Human CDT2 aa 1-200 / 730.

Isotype

IgG

Clone

JE58-01

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, FC

Positive Control

HeLa cell lysate, 293T cell lysate, K-562 cell lysate, Jurkat cell lysate, mouse spleen tissue lysate, HeLa.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 79 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes