TNKS1 Recombinant Rabbit Monoclonal Antibody [PSH0-51]

About this product

Tankyrase, also known as tankyrase 1, is an enzyme that in humans is encoded by the TNKS gene. It inhibits the binding of TERF1 to telomeric DNA. Tankyrase attracts substantial interest in cancer research through its interaction with AXIN1 and AXIN2, which are negative regulators of pro-oncogenic β-catenin signaling. Importantly, activity in the β-catenin destruction complex can be increased by tankyrase inhibitors and thus such inhibitors are a potential therapeutic option to reduce the growth of β-catenin-dependent cancers. Tankyrase-1 is a poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length. Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. May be involved in spindle pole assembly through PARsylation of NUMA1. Stimulates 26S proteasome activity.

Specifications

Catalog number

HA721392

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

ADP-ribosyltransferase diphtheria toxin-like 5 antibody OTTHUMP00000115550 antibody OTTHUMP00000224675 antibody PARP 5a antibody PARP5A antibody PARPL antibody pART5 antibody Poly [ADP-ribose] polymerase 5A antibody TANK 1 antibody TANK1 antibody Tankyrase 1 antibody Tankyrase I antibody Tankyrase TRF1 interacting ankyrin related ADP ribose polymerase antibody Tankyrase-1 antibody Tankyrase1 antibody TankyraseI antibody TIN 1 antibody TIN1 antibody TINF 1 antibody TINF1 antibody TNKS 1 antibody TNKS antibody TNKS-1 antibody TNKS1 antibody TNKS1_HUMAN antibody TRF1 interacting ankyrin related ADP ribose polymerase antibody TRF1-interacting ankyrin-related ADP-ribose polymerase antibody

Abbreviation

TNKS, PARP5A, PARPL, TIN1, TINF1, TNKS1

Swiss Prot

O95271 Human

Cellular Locus

Cytoplasm, Golgi apparatus membrane, cytoskeleton, microtubule organizing center, centrosome, Nucleus, nuclear pore complex, Chromosome, telomere, cytoskeleton, spindle pole.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human TNKS1 aa 151-650/1,327.

Isotype

IgG

Clone

PSH0-51

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB

Positive Control

HeLa cell lysate, Jurkat cell lysate, MCF7 cell lysate, Raji cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate.

Concentration

1 mg/mL

Dilution

WB: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 142 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes