Biotinylated Cynomolgus / Rhesus macaque CTLA-4 Protein, Avitag™, His Tag

About this product

Biotinylated Cynomolgus / Rhesus macaque CTLA-4, Avitag, His Tag (CT4-C82E5) is expressed from human 293 cells (HEK293) . It contains AA Ala 37 - Ser 160 (Accession # G7PL88-1) . In the region Ala 37 - Ser 160, the AA sequence of Cynomolgus and Rhesus macaque CTLA-4 are homologus.

Specifications

Catalog number

CT4-C82E5-200ug

Size / count

200 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.

Specifications

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 15.9 kDa. The protein migrates as 20-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

G7PL88-1

Expression Region

Ala 37 - Ser 160

Host

HEK293

Target

CTLA-4

Conjugation

Biotin-labeled

Tag

C-Avi & C-6xHis

Source

Cynomolgus / Rhesus macaque

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque CTLA-4, Avitag,His Tag (Cat. No. CT4-C82E5) with a linear range of 2-31 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

15.9 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

200ug*1

Host or Source

HEK293

Species

Cynomolgus / Rhesus macaque

Protein ID

G7PL88-1

Preservative

Trehalose