Human Angiopoietin-2 / ANGPT2 Protein, His Tag

About this product

Human Angiopoietin-2, His Tag (AN2-H52H4) is expressed from human 293 cells (HEK293). It contains AA Tyr 19 - Phe 496 (Accession # O15123-1).

Specifications

Catalog number

AN2-H52H4-100ug

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Angiopoietin-2 is also known as ANGPT2, AGPT2, ANG2, and is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies. Both Ang2 and the related Angiopoietin1 (Ang1) are ligands for the receptor tyrosine kinase Tie 2. Ang2 functions as a proangiogenic factor, although it can also induce EC death and vessel regression. Upon its release from quiescent EC, it regulates vascular remodeling by promoting EC survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells. Ang2 is required for postnatal vascular remodeling, and it cooperates with Ang1 during lymphatic vessel development. It mediates the upregulation of ICAM1 and VCAM1 on EC, which facilitates the adhesion of leukocytes during inflammation. Ang2 competitively inhibit Ang1-induced endothelial cell responses mediated by Tie2, and reduces vascular integrity. But the role of Ang2 is controversial since the opposite outcomes has been reported in other studies. Over-expression of Ang2 disrupts the vascular remodeling, induce endothelial cell apoptosis, and may play an important regulating role in tumor angiogenesis. Ang2 also promotes the neuronal differentiation and migration of subventricular zone progenitor cells.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 56.8 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Tyr 19 - Phe 496

Host

HEK 293

Target

Angiopoietin-2

Conjugation

Unconjugated

Tag

C-10×His

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human Angiopoietin-2, His Tag (Cat. No. AN2-H52H4) at 2 μg/mL (100 μL/well) can bind Human TIE2, Fc Tag (Cat. No. TI2-H5255) with a linear range of 1-39 ng/mL (QC tested).

Format

Powder

Buffer

20 mM MOPS, 150 mM NaCl, pH7.5

Additives

Trehalose

Molecular Weight

56.8 kDa

Shipping Conditions

Room Temperature

Storage Conditions

-20°C