Human Siglec-3 / CD33 Protein, Llama IgG2b Fc Tag, low endotoxin

About this product

Human Siglec-3 Protein, Llama IgG2b Fc Tag (CD3-H5259) is expressed from human 293 cells (HEK293) . It contains AA Asp 18 - His 259 (Accession # AAH28152.1) .

Specifications

Catalog number

CD3-H5259-100ug

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase (s) via their SH2 domain (s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.

Specifications

This protein carries a llama IgG2b Fc tag at the C-terminus. The protein has a calculated MW of 54.6 kDa. The protein migrates as 64-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAH28152.1

Expression Region

Asp 18 - His 259

Host

HEK293

Target

Siglec-3

Conjugation

Unconjugated

Tag

C-LlamaFc (LlamaIgG2b)

Source

Human

Stability

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

95%

Bioactivity

Immobilized Human Siglec-3 Protein, Llama IgG2b Fc Tag (Cat. No. CD3-H5259) at 5 μg/mL (100 μL/well) can bind Anti-CD33, Human IgG1 with a linear range of 0.078-1.25 μg/mL (QC tested).

Format

Powder

Buffer

Tris with Glycine, Arginine and NaCl, pH7.5

Additives

Trehalose

Molecular Weight

54.6 kDa

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

100ug*1

Species

Human