Human APP / Abeta40 Protein, His Tag

About this product

Human Abeta40, His Tag (APP-H51H7) is expressed from E. coli cells. It contains AA Asp 672 - Val 711 (Accession # P05067-1).

Specifications

Catalog number

APP-H51H7-100ug

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Amyloid precursor protein (APP) is a type I integral membrane protein ubiquitously expressed in many tissues and concentrated in the synapses of neurons. It has three predominant splice variants: APP695, APP751, and APP770. The majority of APP is cleaved at the plasma membrane by the α-secretase in the non-amyloidogenic pathway. The amyloidogenic pathway starts with β-secretase cleavage by BACE1 on the N-terminal part of the Aβ domain, releasing sAPPβ from a membrane-anchored fragment named βCTF or C99, which is subsequently cleaved by γ-secretase to release Aβ.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 6.3 kDa. The protein migrates as 11 kDa under reducing (R) condition (SDS-PAGE).

Expression Region

Asp 672 - Val 711

Host

E. coli

Target

APP

Conjugation

Unconjugated

Tag

C-10×His

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human Abeta40, His Tag (Cat. No. APP-H51H7) at 5 μg/mL (100 μL/well) can bind Aducanumab with a linear range of 0.2-2 ng/mL (QC tested).

Format

Powder

Buffer

PBS, 0.2 M Arginine, pH7.4

Additives

Trehalose

Molecular Weight

6.3 kDa

Shipping Conditions

Room Temperature

Storage Conditions

-20°C