Rabbit CTLA-4 / CD152 Protein, Fc Tag (MALS verified)

About this product

Rabbit CTLA-4, Fc Tag (CT4-R5250) is expressed from human 293 cells (HEK293). It contains AA Lys 36 - Asp 161 (Accession # P42072).

Specifications

Catalog number

CT4-R5250-1mg

Size / count

1 mg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 40.3 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Lys 36 - Asp 161

Host

HEK 293

Target

CTLA-4

Conjugation

Unconjugated

Tag

C-Fc

Source

Rabbit

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human B7-1, mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) at 5 μg/mL (100 μL/well)can bind Rabbit CTLA-4, Fc Tag (Cat. No. CT4-R5250) with a linear range of 0.1-0.8 ng/mL (QC tested).

Format

Powder

Buffer

Tris with Glycine, Arginine and NaCl, pH7.5

Additives

Trehalose

Molecular Weight

40.3 kDa

Shipping Conditions

Room Temperature

Storage Conditions

-20°C