Biotinylated Human IL-2 Protein, His,Avitag™

About this product

Biotinylated Human IL-2, His,Avitag (IL2-H82E4) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 (Accession # P60568-1).

Specifications

Catalog number

IL2-H82E4-25ug

Size / count

25 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 19.1 kDa. The protein migrates as 19 kDa and 20 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Ala 21 - Thr 153

Host

HEK 293

Target

IL-2

Conjugation

Biotin-labeled

Tag

C-10×His & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human IL-2 R alpha, Fc Tag (Cat. No. ILA-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2, His,Avitag (Cat. No. IL2-H82E4) with a linear range of 0.6-5 ng/mL (QC tested).

Format

Powder

Buffer

PBS with Arginine, pH7.4

Additives

Trehalose

Molecular Weight

19.1 kDa

Shipping Conditions

Room Temperature

Storage Conditions

-20°C