Human IL-7-P2A Protein, Tag Free

About this product

Human IL-7-P2A, Tag Free (ILA-H5218) is expressed from human 293 cells (HEK293). It contains AA Asp 26 - His 177 (Accession # P13232-1).

Specifications

Catalog number

ILA-H5218-50ug

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Interleukin 7 is also known as IL7, IL-7, and is a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells, but is not produced by lymphocytes. IL-7 stimulates the differentiation of multipotent (pluripotent) hematopoietic stem cells into lymphoid progenitor cells, It also stimulates proliferation of all cells in the lymphoid lineage (B cells, T cells and NK cells). It is important for proliferation during certain stages of B-cell maturation, T and NK cell survival, development and homeostasis. IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL-7 binds to the IL-7 receptor, a heterodimer consisting of Interleukin-7 receptor alpha and common gamma chain receptor. Il-7 promotes hematological malignacies (acute lymphoblastic leukemia, T cell lymphoma). Elevated levels of IL-7 have also been detected in the plasma of HIV-infected patients. IL-7 as an immunotherapy agent has been examined in many pre-clinical animal studies and more recently in human clinical trials for various malignancies and during HIV infection. IL-7 could also be beneficial in improving immune recovery after allogenic stem cell transplant.

Specifications

This protein carries no "tag". The protein has a calculated MW of 19.6 kDa. The protein migrates as 26-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Asp 26 - His 177

Host

HEK 293

Target

IL-7-P2A

Conjugation

Unconjugated

Tag

Native

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human IL-7-P2A, Tag Free (Cat. No. ILA-H5218) at 2 μg/mL (100 μL/well) can bind Human IL-7 R alpha, Fc Tag with a linear range of 0.6-39 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

19.6 kDa

Shipping Conditions

Room Temperature

Storage Conditions

-20°C