Mouse TIGIT Protein, Mouse IgG2a Fc Tag (MALS verified)

Catalog number TIT-M5253-50ug

Discontinued

Price for TIT-M5253-50ug

Size / count 50 µg

This variant is discontinued and cannot be added to your cart.

About this product

Mouse TIGIT, Mouse IgG2a Fc Tag (TIT-M5253) is expressed from human 293 cells (HEK293). It contains AA Gly 26 - Thr 143 (Accession # NP_001139797.1).

Specifications

Catalog number: TIT-M5253-50ug
Size / count: 50 µg
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Specifications: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 39.8 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Gly 26 - Thr 143
Host: HEK 293
Target: TIGIT
Conjugation: Unconjugated
Tag: C-mFc(mIgG2a)
Source: Mouse
Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin: 0.1 EU per μg
Purity: 95%
Bioactivity: Immobilized Biotinylated Mouse CD155, Fc,Avitag (Cat. No. CD5-M82F7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse TIGIT, Mouse IgG2a Fc Tag (Cat. No. TIT-M5253) with a linear range of 0.1-2 ng/mL (QC tested).
Format: Powder
Buffer: PBS, pH7.4
Additives: Trehalose
Molecular Weight: 39.8 kDa
Shipping Conditions: Room Temperature
Storage Conditions: -20°C

Frequently Asked Questions

What is the catalog number for Mouse TIGIT Protein, Mouse IgG2a Fc Tag (MALS verified)?

The catalog number for Mouse TIGIT Protein, Mouse IgG2a Fc Tag (MALS verified) is TIT-M5253-50ug.

Is Mouse TIGIT Protein, Mouse IgG2a Fc Tag (MALS verified) in stock?

Mouse TIGIT Protein, Mouse IgG2a Fc Tag (MALS verified) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Mouse TIGIT Protein, Mouse IgG2a Fc Tag (MALS verified)?

Mouse TIGIT Protein, Mouse IgG2a Fc Tag (MALS verified) is manufactured by CRB.

What is the estimated delivery time for Mouse TIGIT Protein, Mouse IgG2a Fc Tag (MALS verified)?

Estimated delivery time for Mouse TIGIT Protein, Mouse IgG2a Fc Tag (MALS verified) is approximately 10 business day(s), depending on your location.

What is Mouse TIGIT Protein, Mouse IgG2a Fc Tag (MALS verified) used for?

Mouse TIGIT, Mouse IgG2a Fc Tag (TIT-M5253) is expressed from human 293 cells (HEK293). It contains AA Gly 26 - Thr 143 (Accession # NP_001139797.1).

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.