Biotinylated Human DLL3 Protein, His,Avitag™ (MALS verified)

About this product

Biotinylated Human DLL3, His,Avitag (DL3-H82E4) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Leu 492 (Accession # Q9NYJ7-1).

Specifications

Catalog number

DL3-H82E4-25ug

Size / count

25 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands.May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. DLL3 protein is expressed on the surface of tumor cells but not in normal adult tissues.

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 52.1 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Ala 27 - Leu 492

Host

HEK 293

Target

DLL3

Conjugation

Biotin-labeled

Tag

C-10×His & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Biotinylated Human DLL3, His,Avitag (Cat. No. DL3-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-DLL3 Antibody (specific Binding EGFs of DLL3), Human IgG1 with a linear range of 0.1-4 ng/mL (QC tested).

Format

Powder

Buffer

PBS, 0.2 M Arginine, pH7.4

Additives

Trehalose

Molecular Weight

52.1 kDa

Shipping Conditions

Room Temperature

Storage Conditions

-20°C