Rat MCAM / CD146 Protein, His Tag

Catalog number MCM-R52H4-100ug

Discontinued

Price for MCM-R52H4-100ug

Size / count 100 µg

This variant is discontinued and cannot be added to your cart.

About this product

Rat MCAM, His Tag (MCM-R52H4) is expressed from human 293 cells (HEK293). It contains AA Val 24 - Leu 560 (Accession # Q9EPF2-1).

Specifications

Catalog number: MCM-R52H4-100ug
Size / count: 100 µg
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: CD146, also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. As a member of the Immunoglobulin superfamily, It is expressed on chicken embryonic spleen and thymus, activated human T cells, endothelial progenitors such as angioblasts and mesenchymal stem cells, and strongly expressed on blood vessel endothelium and smooth muscle. CD146 has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. The CD146+ T cells display an immunophenotype consistent with effector memory cells and have a distinct gene profile from the CD146- T cells. As a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor,CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis or immune recognition.
Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 61.2 kDa. The protein migrates as 70-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Val 24 - Leu 560
Host: HEK 293
Target: MCAM
Conjugation: Unconjugated
Tag: C-10×His
Source: Rat
Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin: 1.0 EU per μg
Purity: 95%
Format: Powder
Buffer: PBS, pH7.4
Additives: Trehalose
Molecular Weight: 61.2 kDa
Shipping Conditions: Room Temperature
Storage Conditions: -20°C

Frequently Asked Questions

What is the catalog number for Rat MCAM / CD146 Protein, His Tag?

The catalog number for Rat MCAM / CD146 Protein, His Tag is MCM-R52H4-100ug.

Is Rat MCAM / CD146 Protein, His Tag in stock?

Rat MCAM / CD146 Protein, His Tag is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Rat MCAM / CD146 Protein, His Tag?

Rat MCAM / CD146 Protein, His Tag is manufactured by CRB.

What is the estimated delivery time for Rat MCAM / CD146 Protein, His Tag?

Estimated delivery time for Rat MCAM / CD146 Protein, His Tag is approximately 10 business day(s), depending on your location.

What is Rat MCAM / CD146 Protein, His Tag used for?

Rat MCAM, His Tag (MCM-R52H4) is expressed from human 293 cells (HEK293). It contains AA Val 24 - Leu 560 (Accession # Q9EPF2-1).

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.