Sus scrofa CD73 / NT5E Protein, His Tag (active enzyme, MALS verified)

About this product

Sus scrofa CD73, His Tag (CD3-S52H3) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549 (Accession # XP_001927130.1).

Specifications

Catalog number

CD3-S52H3-100ug

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 59.5 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Trp 27 - Ser 549

Host

HEK 293

Target

CD73

Conjugation

Unconjugated

Tag

C-10×His

Source

Sus scrofa (Pig)

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Format

Powder

Buffer

20 mM Tris, 120 mM NaCl, pH7.5

Additives

Trehalose

Molecular Weight

59.5 kDa

Shipping Conditions

Room Temperature

Storage Conditions

-20°C