Human SIRP alpha / CD172a Protein, Llama IgG2b Fc Tag, low endotoxin (MALS verified)

About this product

Human SIRP alpha, Llama IgG2b Fc Tag (SIA-H5256) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # P78324-1).

Specifications

Catalog number

SIA-H5256-100ug

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

Specifications

This protein carries a llama IgG2b Fc tag at the C-terminus. The protein has a calculated MW of 65.1 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Glu 31 - Arg 370

Host

HEK 293

Target

SIRP alpha

Conjugation

Unconjugated

Tag

C-LlamaFc(LlamaIgG2b)

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

95%

Bioactivity

Immobilized Human SIRP alpha, Llama IgG2b Fc Tag (Cat. No. SIA-H5256) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) with a linear range of 0.2-16 ng/mL (QC tested).

Format

Powder

Buffer

50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5

Additives

Trehalose

Molecular Weight

65.1 kDa

Shipping Conditions

Room Temperature

Storage Conditions

-20°C