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Product details

SARS S1 protein, His Tag (MALS verified)

Catalog number S1N-S52H5-100ug

Discontinued

Price for S1N-S52H5-100ug

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Size / count 100 µg

This variant is discontinued and cannot be added to your cart.

About this product

SARS S1 protein, His Tag (S1N-S52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Arg 667 (Accession # AAP13567.1).

Specifications

Catalog number
S1N-S52H5-100ug
Size / count
100 µg
Dry ice
No
Hazardous
No
Discontinued
Yes
Background
The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 74.9 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region
Ser 14 - Arg 667
Host
HEK 293
Target
Spike S1
Conjugation
Unconjugated
Tag
C-10×His
Source
SARS
Stability
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Bioactivity
Immobilized SARS S1 protein, His Tag (Cat. No. S1N-S52H5) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.4-8 ng/mL (QC tested).
Format
Powder
Buffer
PBS, 0.2 M Sodium citrate, pH5.3
Additives
Trehalose
Molecular Weight
74.9 kDa
Shipping Conditions
Room Temperature
Storage Conditions
-20°C

Frequently Asked Questions

What is the catalog number for SARS S1 protein, His Tag (MALS verified)?

The catalog number for SARS S1 protein, His Tag (MALS verified) is S1N-S52H5-100ug.

Is SARS S1 protein, His Tag (MALS verified) in stock?

SARS S1 protein, His Tag (MALS verified) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures SARS S1 protein, His Tag (MALS verified)?

SARS S1 protein, His Tag (MALS verified) is manufactured by CRB.

What is the estimated delivery time for SARS S1 protein, His Tag (MALS verified)?

Estimated delivery time for SARS S1 protein, His Tag (MALS verified) is approximately 10 business day(s), depending on your location.

What is SARS S1 protein, His Tag (MALS verified) used for?

SARS S1 protein, His Tag (S1N-S52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Arg 667 (Accession # AAP13567.1).

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

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