SARS-CoV-2 Nucleocapsid protein (R203M, D377Y), His Tag

Catalog number NUN-C52Hn-100ug

Discontinued

Price for NUN-C52Hn-100ug

Size / count 100 µg

This variant is discontinued and cannot be added to your cart.

About this product

SARS-CoV-2 Nucleocapsid protein (R203M, D377Y), His Tag (NUN-C52Hn) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (R203M, D377Y)). The mutations (R203M, D377Y) on nucleocapsid protein were identified in the SARS-CoV-2 Kappa variant (Pango lineage: B.1.617.1; other names: 21A/S:154K).

Specifications

Catalog number: NUN-C52Hn-100ug
Size / count: 100 µg
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.3 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Met 1 - Ala 419
Host: HEK 293
Target: Nucleocapsid protein
Conjugation: Unconjugated
Tag: C-6×His
Source: SARS-CoV-2
Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin: 1.0 EU per μg
Purity: 95%
Bioactivity: Immobilized SARS-CoV-2 Nucleocapsid protein (R203M, D377Y), His Tag (Cat. No. NUN-C52Hn) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 with a linear range of 0.2-1 ng/mL (QC tested).
Format: Powder
Buffer: PBS, 0.2 M Arginine, pH7.4
Additives: Trehalose
Molecular Weight: 47.3 kDa
Shipping Conditions: Room Temperature
Storage Conditions: -20°C

Frequently Asked Questions

What is the catalog number for SARS-CoV-2 Nucleocapsid protein (R203M, D377Y), His Tag?

The catalog number for SARS-CoV-2 Nucleocapsid protein (R203M, D377Y), His Tag is NUN-C52Hn-100ug.

Is SARS-CoV-2 Nucleocapsid protein (R203M, D377Y), His Tag in stock?

SARS-CoV-2 Nucleocapsid protein (R203M, D377Y), His Tag is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures SARS-CoV-2 Nucleocapsid protein (R203M, D377Y), His Tag?

SARS-CoV-2 Nucleocapsid protein (R203M, D377Y), His Tag is manufactured by CRB.

What is the estimated delivery time for SARS-CoV-2 Nucleocapsid protein (R203M, D377Y), His Tag?

Estimated delivery time for SARS-CoV-2 Nucleocapsid protein (R203M, D377Y), His Tag is approximately 10 business day(s), depending on your location.

What is SARS-CoV-2 Nucleocapsid protein (R203M, D377Y), His Tag used for?

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

Is this product classed as hazardous or restricted for shipping?

Some products are subject to special import/export regulations, dangerous goods handling, or licensing requirements. Where this applies, it’s noted on the product page, and any required extra documents or fees will be confirmed with you before dispatch, never added without your consent.