SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag

Catalog number NUN-C52Hs-100ug

Discontinued

Price for NUN-C52Hs-100ug

Size / count 100 µg

This variant is discontinued and cannot be added to your cart.

About this product

SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag (NUN-C52Hs) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (D63G, R203M, D377Y )). The mutations (D63G, R203M, D377Y) were identified in the SARS-CoV-2 Delta lineages (Pango lineage: B.1.617.2; GISAID clade: 21A, 21I, 21J; Nextstrain clade: G/478K.V1).

Specifications

Catalog number: NUN-C52Hs-100ug
Size / count: 100 µg
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.3 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Met 1 - Ala 419
Host: HEK 293
Target: Nucleocapsid protein
Conjugation: Unconjugated
Tag: C-6×His
Source: SARS-CoV-2
Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin: 1.0 EU per μg
Purity: 95%
Bioactivity: Immobilized SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag (Cat. No. NUN-C52Hs) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 with a linear range of 0.1-1 ng/mL (QC tested).
Format: Powder
Buffer: PBS,pH7.3 with 0.2 M Arginine
Additives: Trehalose
Molecular Weight: 47.3 kDa
Shipping Conditions: Room Temperature
Storage Conditions: -20°C

Frequently Asked Questions

What is the catalog number for SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag?

The catalog number for SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag is NUN-C52Hs-100ug.

Is SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag in stock?

SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag?

SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag is manufactured by CRB.

What is the estimated delivery time for SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag?

Estimated delivery time for SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag is approximately 10 business day(s), depending on your location.

What is SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag used for?

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.