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Product details

SARS-CoV-2 Nucleocapsid protein, His Tag (BA.3*(S33V)/Omicron)

Catalog number NUN-C52Hv-100ug

Discontinued

Price for NUN-C52Hv-100ug

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Size / count 100 µg

This variant is discontinued and cannot be added to your cart.

About this product

SARS-CoV-2 Nucleocapsid protein, His Tag (BA.3*(S33V)/Omicron) (NUN-C52Hv) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (P13L, GER 30-32del, S33V, R203K, G204R, S413R)). The mutations (P13L, GER 30-32del, S33V, R203K, G204R, S413R) were identified in the SARS-CoV-2 Omicron variant (Pango lineage: BA.3; GISAID clade: GRA; Nextstrain clade: 21M).

Specifications

Catalog number
NUN-C52Hv-100ug
Size / count
100 µg
Dry ice
No
Hazardous
No
Discontinued
Yes
Background
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.1 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region
Met 1 - Ala 419
Host
HEK 293
Target
Nucleocapsid protein
Conjugation
Unconjugated
Tag
C-6×His
Source
SARS-CoV-2
Stability
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (BA.3*(S33V)/Omicron) (Cat. No. NUN-C52Hv) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (Cat. No. NUN-CH15) with a linear range of 0.5-2 ng/mL (QC tested).
Format
Powder
Buffer
PBS, 0.2M Arginine, pH7.4
Additives
Trehalose
Molecular Weight
47.1 kDa
Shipping Conditions
Room Temperature
Storage Conditions
-20°C

Frequently Asked Questions

What is the catalog number for SARS-CoV-2 Nucleocapsid protein, His Tag (BA.3*(S33V)/Omicron)?

The catalog number for SARS-CoV-2 Nucleocapsid protein, His Tag (BA.3*(S33V)/Omicron) is NUN-C52Hv-100ug.

Is SARS-CoV-2 Nucleocapsid protein, His Tag (BA.3*(S33V)/Omicron) in stock?

SARS-CoV-2 Nucleocapsid protein, His Tag (BA.3*(S33V)/Omicron) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures SARS-CoV-2 Nucleocapsid protein, His Tag (BA.3*(S33V)/Omicron)?

SARS-CoV-2 Nucleocapsid protein, His Tag (BA.3*(S33V)/Omicron) is manufactured by CRB.

What is the estimated delivery time for SARS-CoV-2 Nucleocapsid protein, His Tag (BA.3*(S33V)/Omicron)?

Estimated delivery time for SARS-CoV-2 Nucleocapsid protein, His Tag (BA.3*(S33V)/Omicron) is approximately 10 business day(s), depending on your location.

What is SARS-CoV-2 Nucleocapsid protein, His Tag (BA.3*(S33V)/Omicron) used for?

SARS-CoV-2 Nucleocapsid protein, His Tag (BA.3*(S33V)/Omicron) (NUN-C52Hv) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (P13L, GER 30-32del, S33V, R203K, G204R, S413R)). The mutations (P13L, GER 30-32del, S33V, R203K, G204R, S413R) were identifie

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

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