BtRf-BetaCoV/JL2012 Spike Trimer Protein (R648A, KV949-950PP), His Tag

About this product

BtRf-BetaCoV/JL2012 Spike Trimer, His Tag (SPN-S52Hs) is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. It contains AA Tyr 16 - Pro 1176 (Accession # A0A0U1UYX4-1 (R648A, KV949-950PP)).

Specifications

Catalog number

SPN-S52Hs-50ug

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 133.3 kDa. The protein migrates as 160-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Tyr 16 - Pro 1176

Host

HEK 293

Target

Spike protein

Conjugation

Unconjugated

Tag

C-10×His

Source

BtRf-BetaCoV/JL2012

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Format

Powder

Buffer

0.1 M Sodium citrate, pH5.5

Additives

Trehalose

Molecular Weight

133.3 kDa

Shipping Conditions

Room Temperature

Storage Conditions

-20°C