Biotinylated Bovine FcRn / FCGRT&B2M Heterodimer Protein, His,Avitag™ (MALS & SPR verified)

About this product

MABSol® Biotinylated Bovine FcRn Heterodimer Protein, His,Avitag (FCN-B82W3) is expressed from human HEK293 cells. It contains AA Ala 24 - Pro 298 (FCGRT) & Ile 21 - Leu 118 (B2M) (Accession # Q3T119-1 (FCGRT) & P01888-1 (B2M)).

Specifications

Catalog number

FCN-B82W3-25ug

Size / count

25 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

Specifications

Biotinylated Bovine FcRn Heterodimer Protein, His,Avitag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.5 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit B2M contains no tag. The reducing (R) protein migrates as 35-38 kDa (FCGRT) and 12 kDa (B2M) respectively due to glycosylation.

Expression Region

Ala 24 - Pro 298 (FCGRT) & Ile 21 - Leu 118 (B2M)

Host

HEK 293

Target

FcRn (FCGRT & B2M)

Conjugation

Biotin-labeled

Tag

C-10×His & C-Avi | Native

Source

Bovine

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

34.5 kDa (FCGRT) and 11.6 kDa (B2M)

Shipping Conditions

Room Temperature

Storage Conditions

-20°C