Rat B7-H2 / ICOSLG Protein, Fc Tag

Catalog number B72-R5259-100ug

Discontinued

Price for B72-R5259-100ug

Size / count 100 µg

This variant is discontinued and cannot be added to your cart.

About this product

Rat B7-H2, Fc Tag (B72-R5259) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Lys 261 (Accession # XP_006256322.1).

Specifications

Catalog number: B72-R5259-100ug
Size / count: 100 µg
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: ICOS ligand (ICOSLG) is also known as B7 homolog 2 (B7-H2), B7-related protein 1 (B7RP-1) and CD antigen CD275, which belongs to the immunoglobulin superfamily and BTN/MOG family. ICOSLG contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. Isoform 1 is widely expressed, while isoform 2 is detected only in lymph nodes, leukocytes and spleen. B7-H2 is ligand for the T-cell-specific cell surface receptor ICOS. B7-H2 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces also B-cell proliferation and differentiation into plasma cells. B7-H2 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
Specifications: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 53.4 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Glu 25 - Lys 261
Host: HEK 293
Target: B7-H2
Conjugation: Unconjugated
Tag: C-Fc
Source: Rat
Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin: 1.0 EU per μg
Purity: 90%
Bioactivity: Immobilized Mouse ICOS (C137S, C138S), His Tag (Cat. No. ICS-M52H8) at 5 μg/mL (100 μL/well) can bind Rat B7-H2, Fc Tag (Cat. No. B72-R5259) with a linear range of 0.6-39 ng/mL (QC tested).
Format: Powder
Buffer: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
Additives: Trehalose
Molecular Weight: 53.4 kDa
Shipping Conditions: Room Temperature
Storage Conditions: -20°C

Frequently Asked Questions

What is the catalog number for Rat B7-H2 / ICOSLG Protein, Fc Tag?

The catalog number for Rat B7-H2 / ICOSLG Protein, Fc Tag is B72-R5259-100ug.

Is Rat B7-H2 / ICOSLG Protein, Fc Tag in stock?

Rat B7-H2 / ICOSLG Protein, Fc Tag is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Rat B7-H2 / ICOSLG Protein, Fc Tag?

Rat B7-H2 / ICOSLG Protein, Fc Tag is manufactured by CRB.

What is the estimated delivery time for Rat B7-H2 / ICOSLG Protein, Fc Tag?

Estimated delivery time for Rat B7-H2 / ICOSLG Protein, Fc Tag is approximately 10 business day(s), depending on your location.

What is Rat B7-H2 / ICOSLG Protein, Fc Tag used for?

Rat B7-H2, Fc Tag (B72-R5259) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Lys 261 (Accession # XP_006256322.1).

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.