Canine PDGF R beta / CD140b Protein, His Tag

About this product

Canine PDGF R beta Protein, His Tag (PDB-C52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 33 - Val 532 (Accession # Q6QNF3-1).

Specifications

Catalog number

PDB-C52H3-1mg

Size / count

1 mg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB) is also called J03278, M21616, CD140B, JTK12, PDGF-R-beta and PDGFR, is receptor that binds specifically to PDGFB and has a tyrosine-protein kinase activity. Is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor (PDGF) family. The PDGFR/PDGF system includes two receptors (PDGFRA and PDGFRB) and four ligands (A, B, C and D). The receptors PDGFRA and PDGFRB are related in sequence and both are members of the class III subtype of receptor tyrosine kinases (RTKs). Other class III RTKs are CSF1R, KIT and FLT3. PDGF binding induces receptor homo- and heterodimerization and signal transduction. The expression of the α and β receptors is independently regulated in various cell types. Recombinant soluble PDGFRB binds PDGF with high affinity and is potent PDGF antagonist. The ligands form either homo- or heterodimers (PDGF-AA, -AB, -BB, -CC, -DD). The four PDGFs are inactive in their monomeric forms. The PDGFs bind to the protein tyrosine kinase receptors PDGF receptor-α and -β. These two receptor isoforms dimerize upon binding the PDGF dimer, leading to three possible receptor combinations, namely -αα, -ββ and -αβ. PDGF-CC specifically interacts with PDGFR-αα and -αβ, but not with –ββ, and thereby resembles PDGF-AB. PDGF-DD binds to PDGFR-ββ with high affinity, and to PDGFR-αβ to a much lower extent and is regarded as PDGFR-ββ specific. PDGF-AA binds only to PDGFR-αα, while PDGF-BB is the only PDGF that can bind all three receptor combinations with high affinity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 58.0 kDa. The protein migrates as 70-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Leu 33 - Val 532

Host

HEK 293

Target

PDGF R beta

Conjugation

Unconjugated

Tag

C-10×His

Source

Canine

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human PDGF-BB, premium grade (Cat. No. PDB-H4112) at 1 μg/mL (100 μL/well) can bind Canine PDGF R beta / CD140b, His Tag (Cat. No. PDB-C52H3) with a linear range of 2-78 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

58.0 kDa

Shipping Conditions

Room Temperature

Storage Conditions

-20°C