SARS-CoV-2 3C-like Proteinase Protein, Tag Free (active enzyme, MALS verified)

About this product

SARS-CoV-2 3C-like Proteinase Protein, Tag Free (3CE-C5113) is expressed from E. coli cells. It contains AA Ser 3264 - Gln 3569 (Accession # P0DTC1-1) .

Specifications

Catalog number

3CE-C5113-100ug

Size / count

100 µg

Dry ice

Yes

Hazardous

No

Discontinued

Yes

Background

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19) . Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.

Specifications

This protein carries no "tag". The protein has a calculated MW of 33.9 kDa. The protein migrates as 33-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .

Accession Number

P0DTC1-1

Expression Region

Ser 3264 - Gln 3569

Host

E. coli

Target

3C-like Proteinase

Conjugation

Unconjugated

Tag

Native

Source

SARS-CoV-2

Stability

● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

95%

Format

Liquid

Buffer

50 mM Tris, pH7.3

Additives

Glycerol

Molecular Weight

33.9 kDa

Shipping Conditions

Dry ice

Storage Conditions

-70°C

Package Size

100ug*1

Species

SARS-CoV-2