Human Claudin-1 Protein, Flag, His Tag (Detergent)

About this product

Human Claudin-1 Protein, Flag, His Tag (CL1-H55D8) is expressed from Baculovirus-Insect cells. It contains AA Ala 2 - Val 211 (Accession # O95832) .

Specifications

Catalog number

CL1-H55D8-500ug

Size / count

500 µg

Dry ice

Yes

Hazardous

No

Discontinued

Yes

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008]

Specifications

This protein carries a flag tag at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 25.8 kDa. The protein migrates as 8 kDa, 10 kDa, 20 kDa and 22-23 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .

Accession Number

O95832

Expression Region

Ala 2 - Val 211

Host

Insect Cells

Target

Claudin-1

Conjugation

Unconjugated

Tag

N-Flag & N-10xHis

Source

Human

Stability

● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 12 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human Claudin-1 Protein, Flag,His Tag (Cat. No. CL1-H55D8) at 5 μg/mL (100 μL/well) on a Nickel Coated plate can bind Monoclonal Anti-Human Claudin-1 antibody, Human IgG1 with a linear range of 0.001-0.625 μg/mL (QC tested).

Format

Liquid

Buffer

50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5

Additives

Glycerol

Molecular Weight

25.8 kDa

Shipping Conditions

Dry ice

Storage Conditions

-70°C

Package Size

500ug*1

Species

Human