Cyclin D1 Rabbit Polyclonal Antibody

About this product

Cyclin D1 is a protein that in humans is encoded by the CCND1 gene. The CCND1 gene encodes the cyclin D1 protein. The human CCND1 gene is located on the long arm of chromosome 11 (band 11q13). It is 13,388 base pairs long, and translates into 295 amino acids. Cyclin D1 is expressed in all adult human tissues with the exception of cells derived from bone marrow stem cell lines (both lymphoid and myeloid). The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDKs (Cyclin-dependent kinase). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Micrograph of cyclin D1 staining in a mantle cell lymphoma. Immunohistochemical staining of cyclin D1 antibodies is used to diagnose mantle cell lymphoma. Cyclin D1 has been found to be overexpressed in breast carcinoma. Its potential use as a biomarker was suggested.

Specifications

Catalog number

0407-27

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

AI327039 antibody B cell CLL/lymphoma 1 antibody B cell leukemia 1 antibody B cell lymphoma 1 protein antibody B-cell lymphoma 1 protein antibody BCL 1 antibody BCL-1 antibody BCL-1 oncogene antibody BCL1 antibody BCL1 oncogene antibody ccnd1 antibody cyclind1 antibody CCND1/FSTL3 fusion gene, included antibody CCND1/IGHG1 fusion gene, included antibody CCND1/IGLC1 fusion gene, included antibody CCND1/PTH fusion gene, included antibody CCND1_HUMAN antibody cD1 antibody Cyl 1 antibody D11S287E antibody G1/S specific cyclin D1 antibody G1/S-specific cyclin-D1 antibody Parathyroid adenomatosis 1 antibody PRAD1 antibody PRAD1 oncogene antibody U21B31 antibody

Abbreviation

CCND1

Swiss Prot

P24385 Human, P25322 Mouse, P39948 Rat

Cellular Locus

Nucleus, Cytoplasm, Nucleus membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Zebrafish

Immunogen

This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of C-terminal residues of cyclin D1.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IF-Cell, FC

Positive Control

MCF-7 lysates, A549, HepG2, N2A, A431.

Concentration

1 mg/mL

Dilution

WB: 1:5,000 ;IF-Cell: 1:200 ;FC: 1:500-1:1,000

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 34 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No