beta Tubulin Mouse Monoclonal Antibody [1-B11]

About this product

Tubulin in molecular biology can refer either to the tubulin protein superfamily of globular proteins, or one of the member proteins of that superfamily. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes, including mitosis. Tubulin-binding drugs kill cancerous cells by inhibiting microtubule dynamics, which are required for DNA segregation and therefore cell division. In eukaryotes, there are six members of the tubulin superfamily, although not all are present in all species. Both α and β tubulins have a mass of around 50 kDa and are thus in a similar range compared to actin (with a mass of ~42 kDa). In contrast, tubulin polymers (microtubules) tend to be much bigger than actin filaments due to their cylindrical nature. Tubulin was long thought to be specific to eukaryotes. More recently, however, several prokaryotic proteins have been shown to be related to tubulin.

Specifications

Catalog number

EM0103

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Beta 4 tubulin antibody Beta 5 tubulin antibody BetaTubulin antibody TBB5_HUMAN antibody TUBB antibody TUBB2 antibody TUBB2A antibody TUBB5 antibody tubulin beta 2A antibody Tubulin beta chain antibody Tubulin beta-5 chain antibody

Abbreviation

TBB5

Swiss Prot

P07437 Human, P99024 Mouse, P69897 Rat

Cellular Locus

Cytoplasm ,cytoskeleton

Host

Mouse

Species Reactivity

Human, Mouse, Rat, Zebrafish

Immunogen

Synthetic peptide within human Beta tubulin aa 51-100.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC, IF-Tissue

Positive Control

K-562 cell lysate, Jurkat cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Hela, HepG2, NIH/3T3, mouse brain tissue, hybrid fish (crucian-carp) brain tissue lysates.

Concentration

2 mg/mL

Dilution

WB: 1:5,000-1:20,000 ;IF-Cell: 1:1,00 ;IHC-P: 1:3,000-1:10,000 ;FC: 1:50-1:100 ;IF-Tissue: 1:600-1:2,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 50 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No