Vimentin Mouse Monoclonal Antibody [D4-B11]

About this product

Vimentin is a structural protein that in humans is encoded by the VIM gene. Its name comes from the Latin vimentum which refers to an array of flexible rods. Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all animal cells[6] as well as bacteria. Intermediate filaments, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Specifications

Catalog number

EM0401

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

CTRCT30 antibody Epididymis luminal protein 113 antibody FLJ36605 antibody HEL113 antibody VIM antibody VIME_HUMAN antibody Vimentin antibody

Abbreviation

VIME

Swiss Prot

P08670 Human, P20152 Mouse, P31000 Rat

Cellular Locus

Cytoplasm

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within C-terminal human Vimentin.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC, IF-Tissue, IHC-Fr, IP

Positive Control

HeLa cell lysate, C2C12 cell lysate, C6 cell lysate, NIH/3T3 cell lysate, Mouse embryonic stem cell lysate, Hela, human kidney tissue, human colon carcinoma tissue, human stomach carcinoma tissue, human tonsil tissue, human skin tissue, human liver tissue, human appendix tissue, human endometrium tissue.

Concentration

2 mg/mL

Dilution

WB: 1:2,000-1:5,000 ;IF-Cell: 1:200 ;IHC-P: 1:200 ;FC: 1:500-1:1,000 ;IF-Tissue: 200-400 ;IHC-Fr: 1:100 ;IP: Use at an assay dependent concentration

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 54 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No