PCNA Mouse Monoclonal Antibody [A6-G11]

About this product

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Specifications

Catalog number

EM111201

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

ATLD2 antibody cb16 antibody Cyclin antibody DNA polymerase delta auxiliary protein antibody etID36690.10 antibody fa28e03 antibody fb36g03 antibody HGCN8729 antibody MGC8367 antibody Mutagen-sensitive 209 protein antibody OTTHUMP00000030189 antibody OTTHUMP00000030190 antibody PCNA antibody Pcna/cyclin antibody PCNA_HUMAN antibody PCNAR antibody Polymerase delta accessory protein antibody Proliferating cell nuclear antigen antibody wu:fa28e03 antibody wu:fb36g05 antibody

Abbreviation

PCNA

Swiss Prot

P12004 Human, P17918 Mouse, P04961 Rat

Cellular Locus

Nucleus

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human PCNA aa 1-261.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P, FC, IF-Cell

Positive Control

HCT 116 cell lysate, HEK-293 cell lysate, Raji cell lysate, HeLa cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, L-929 cell lysate, C2C12 cell lysate, rat spleen tissue lysate, mouse spleen tissue lysate, human liver tissue lysate, HeLa, human colon cancer tissue, mouse testis tissue, rat testis tissue.

Concentration

2 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IHC-P: 1:10,000 ;FC: 1:1,000 ;IF-Cell: 1:500

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 29 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No