GFAP Mouse Monoclonal Antibody [1-D4]

About this product

Glial fibrillary acidic protein (GFAP) is a protein that is encoded by the GFAP gene in humans. It is a type III intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS), including astrocytes and ependymal cells during development. GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts taken from rat kidneys, Leydig cells of the testis in both hamsters and humans, human keratinocytes, human osteocytes and chondrocytes and stellate cells of the pancreas and liver in rats. GFAP is closely related to the other three non-epithelial type III IF family members, vimentin, desmin and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength as well as the shape of cells, but its exact function remains poorly understood, despite the number of studies using it as a cell marker. There are multiple disorders associated with improper GFAP regulation, and injury can cause glial cells to react in detrimental ways. Glial scarring is a consequence of several neurodegenerative conditions, as well as injury that severs neural material. Another condition directly related to GFAP is Alexander disease, a rare genetic disorder. Notably, the expression of some GFAP isoforms have been reported to decrease in response to acute infection or neurodegeneration. Additionally, reduction in GFAP expression has also been reported in Wernicke's encephalopathy.

Specifications

Catalog number

EM140707

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

wu:fb34h11 antibody ALXDRD antibody cb345 antibody etID36982.3 antibody FLJ42474 antibody FLJ45472 antibody GFAP antibody GFAP_HUMAN antibody gfapl antibody Glial fibrillary acidic protein antibody Intermediate filament protein antibody wu:fk42c12 antibody xx:af506734 antibody zgc:110485 antibody

Abbreviation

GFAP

Swiss Prot

P14136 Human, P03995 Mouse, P47819 Rat

Cellular Locus

Cytoplasm.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within C-terminal human GFAP.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P, IF-Cell, IF-Tissue, FC

Positive Control

Human brain tissue lysate, rat brain tissue lysate, human cerebellum tissue lysate, rat cerebellum tissue lysate, A172, N2A, rat brain tissue, rat cerebellum tissue, Hela, rat hippocampus tissue.

Concentration

2 mg/mL

Dilution

WB: 1:5,000 ;IF-Cell: 1:50-1:500 ;IF-Tissue: 1:500 ;IHC-P: 1:500-1:1,000 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 50 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No