B-Raf Mouse Monoclonal Antibody [F1-F4-E10]

About this product

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.

Specifications

Catalog number

EM1701-32

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

FLJ95109 antibody 94 kDa B raf protein antibody B raf 1 antibody B Raf proto oncogene serine threonine protein kinase antibody B Raf proto oncogene, serine/threonine kinase antibody B RAF1 antibody B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody BRAF 1 antibody BRAF antibody BRAF_HUMAN antibody BRAF1 antibody cRmil antibody MGC126806 antibody MGC138284 antibody Murine sarcoma viral (v-raf) oncogene homolog B1 antibody Murine sarcoma viral v raf oncogene homolog B1 antibody NS7 antibody Oncogen BRAF antibody oncogene BRAF1 antibody p94 antibody Proto-oncogene B-Raf antibody Proto-oncogene c-Rmil antibody RAFB 1 antibody RAFB1 antibody RMIL antibody Serine/threonine-protein kinase B-raf antibody v raf murine sarcoma viral oncogene homolog B antibody v raf murine sarcoma viral oncogene homolog B1 antibody v-Raf murine sarcoma viral oncogene homolog B1 antibody

Abbreviation

BRAF, BRAF1, RAFB1

Swiss Prot

P15056 Human, P28028 Mouse,

Cellular Locus

Nucleus, Cytoplasm, Cell membrane.

Host

Mouse

Species Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Recombinant protein within Human B-Raf aa 49-239 / 766.

Isotype

IgG2b

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P, IF-Cell

Positive Control

HeLa cell lysate, K-562 cell lysate, HT-29 cell lysate, SK-MEL-28 cell lysate, HEK-293 cell lysate, SiHa cell lysate, COS-1 cell lysate, Vero cell lysate, PC-12 cell lysate, LOVO, SH-SY5Y, rat testis tissue, human testis tissue, human colon cancer tissue, human placenta tissue, mouse brain tissue.

Concentration

2 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:100-1:500 ;IHC-P: 1:500-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 84 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No