CD47 Mouse Monoclonal Antibody [1A9]

About this product

CD47 is an integral membrane protein that plays a role in the regulation of cation fluxes across cell membranes. Specifically, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to the extracellular matrix. It is also a receptor for the C-terminal cell binding domain of thrombospondin (SIRP). Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. CD47 is absent from Rh-null erythrocytes, but does play a role in cell adhesion in non-erythroid cells and may prevent premature elimination of erythrocytes. It may also be involved in membrane permeability changes following viral infection. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. CD47 is expressed on hemopoietic cells, epithelial cells, endothelial cells and fibroblasts and is strongly expressed in brain and mesenchymal cells.

Specifications

Catalog number

EM1701-38

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Antigen identified by monoclonal antibody 1D8 antibody Antigenic surface determinant protein OA3 antibody CD 47 antibody Cd47 antibody CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody CD47 antigen antibody CD47 glycoprotein antibody CD47 molecule antibody CD47_HUMAN antibody IAP antibody Integrin Associated Protein antibody Integrin associated signal transducer antibody Integrin-associated protein antibody Leukocyte surface antigen CD47 antibody MER 6 antibody MER6 antibody OA 3 antibody OA3 antibody OTTHUMP00000041152 antibody OTTHUMP00000041153 antibody Protein MER6 antibody Rh related antigen antibody Surface antigen identified by monoclonal antibody 1D8 antibody

Abbreviation

IAP, CD47, MER6

Swiss Prot

Q08722 Human

Cellular Locus

Cell membrane, Multi-pass membrane protein.

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant protein within Human CD47 aa 19-141 (Extracellular).

Isotype

IgM

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

IF-Cell, IHC-P, FC

Positive Control

Human ovarian carcinoma tissue, human prostate tissue, SH-SY5Y.

Concentration

2 mg/mL

Dilution

IF-Cell: 1:50-1:100;IHC-P: 1:200-1,000;FC: 1:50-1:100

Purity

Protein G affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 35 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No