MSH2 Mouse Monoclonal Antibody [10G2]

About this product

Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containg DNA strand. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP>ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Specifications

Catalog number

EM1801-05

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

BAT26 antibody COCA 1 antibody COCA1 antibody DNA mismatch repair protein Msh2 antibody FCC 1 antibody FCC1 antibody hMSH2 antibody HNPCC 1 antibody HNPCC antibody HNPCC1 antibody LCFS2 antibody MSH 2 antibody Msh2 antibody MSH2_HUMAN antibody MutS homolog 2 antibody MutS homolog 2 colon cancer nonpolyposis type 1 antibody MutS protein homolog 2 antibody

Abbreviation

hMSH2, MSH2

Swiss Prot

P43246 Human, P43247 Mouse, P54275 Rat

Cellular Locus

Nucleus, Chromosome.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within N-terminal human MSH2.

Isotype

IgG2b

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P, IF-Tissue

Positive Control

HeLa cell lysate, HEK-293 cell lysate, A549 cell lysate, A431 cell lysate, HCT 116 cell lysate, SW480 cell lysate, PC-3M cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human breast cancer tissue, human colon cancer tissue, human stomach cancer tissue, human appendix tissue, human appendicular lymph nodes tissue, mouse colon tissue, rat colon tissue.

Concentration

2 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IHC-P: 1:1,000 ;IF-Tissue: 1:200

Purity

Protein G affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 105 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No